National Dairy Research Institute, N.D.R.I, Karnal has achieved yet another feat by successfully cloning a male buffalo calf named ‘Shresth’. Scientists of N.D.R.I Karnal made this achievement during early hours today through the new and advanced ‘Hand-guided Cloning Technique’. The hand-guided cloning technique developed at NDRI, is an advanced modification of the “Conventional Cloning Technique”.
This cloned calf weighing 41 kg was born through normal delivery with slight assistance carried out by a team of doctors. This cloned buffalo calf is different from the earlier clone calves as, in this case, the foster mother was provided opportunity for normal delivery, the cloned calf was from ear somatic cell of 2 week old buffalo calf, and the embryo which led to successful pregnancy and normal delivery had remained frozen at -196C for one week in liquid nitrogen and brought back to active life upon thawing at room temperature. The earlier two calves were born through caesarean operation and were produced by using cells from foetus and embryonic stem cell, respectively.
The scientists are of the opinion that the cryopreservation of embryos will need to be made as part of technique, so that the embryos could be transported and used at several places.
Earlier the NDRI has produced the world’s first cloned buffalo calf on 6th February, 2009 followed by second on June 6, 2009 and third on August 22, 2010.
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